Reversion of Bacillus megaterium protoplasts to the bacillary form.
نویسندگان
چکیده
Photomicrographic evidence of reversion of Bacillus megaterium protoplasts to the bacillary form on soft agar plates hypertonic medium is demonstrated.
منابع مشابه
Morphological study of the reversion to bacillary form, of Bacillus megaterium protoplasts.
Protoplasts of Bacillus megaterium readily reverted to bacillary form in liquid media and when plated in a soft-agar layer onto the surface of appropriate agar media. Three phases of the reversion sequence could be differentiated by phase contrast microscopy: (i) increase in size of the individual protoplasts, (ii) non oriented division of the protoplasts and (iii) outgrowth of the bacillary fo...
متن کاملGelatin-induced reversion of protoplasts of Bacillus subtilis to the bacillary form: electron-microscopic and physical study.
Protoplasts of Bacillus subtilis plated on SD medium form L colonies in quantitative yield and propagate in the L form indefinitely. L bodies or protoplasts placed in 25% gelatin medium form bacillary colonies. Details of the reversion of naked bodies to the walled form are reported. In 25% gelatin medium, reversion begins earlier (about 50% reversion in 4 hr) than the multiplication of bacilli...
متن کاملGelatin-induced reversion of protoplasts of Bacillus subtilis to the bacillary form: photomicrographic study.
When lysozyme protoplasts of Bacillus subtilis 168 indoleare washed free of lysozyme and plated on hypertonic soft agar medium, each protoplast forms an L colony. The L colonies consist of cell wall-free L bodies which, in turn, give rise to new L colonies. This heritably propagated wall-less condition can be terminated promptl"'6 by plating the cells in 25%, gelatin medium. In this&t;environme...
متن کاملEnzymically and Physically Induced Inheritance Changes in Bacillus Subtilis.
When a culture of Bacillus subtilis strain 168 is treated with a solution of crystalline lysozyme, the bacteria pass through an osmotically sensitive bacillary phase to the protoplast stage. All of the protoplasts can be recovered as L-colony-forming elements on complex or chemically defined osmotically stabilized agar media. Protoplasts or L-bodies can be propagated in the L-state indefinitely...
متن کاملGelatin-induced reversion of protoplasts of Bacillus subtilis to the bacillary form: biosynthesis of macromolecules and wall during successive steps.
Protoplasts of Bacillus subtilis plated on SDG medium formed L colonies in quantative yield and propagated in the L-form indefinitely. Protoplasts or L bodies placed in 25% gelatin medium formed bacillary colonies. Details of the reversion of these naked bodies to the walled form are reported here. Protoplasts prepared in minimal medium reverted fairly synchronously 3 to 4 hr after inoculation ...
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عنوان ژورنال:
- Journal of bacteriology
دوره 121 1 شماره
صفحات -
تاریخ انتشار 1975